Читать книгу Transfusion Medicine - Jeffrey McCullough - Страница 186

Because of the variability in the number of cells that may be obtained, the strategy for using the cells for transplantation cannot always be the same. If the dose needed for transplantation can be obtained with one procedure, the cells can be transfused immediately. However, if two or three apheresis procedures are necessary, it may be desirable to freeze the concentrates and transfuse them all at once. However, the freezing and thawing may alter the composition of the PBSC concentrates, and so some transplant physicians give the cells fresh each day until the desired dose is obtained. Alternatively, the concentrate collected on the first day is stored in the liquid state and transfused with the concentrate collected on the second day. It appears that PBSCs can be preserved satisfactorily in Plasmalyte A, Normosol or STM‐Sav for 24 hours at room temperature [125]. A more extensive discussion of hematopoietic stem cell preservation is provided in Chapter 19.

Figure 6.3 CD34+ cell yield in peripheral blood concentrates collected from normal donors.

(Source: Reproduced with permission from Stroncek DF, Clay ME, Smith J, et al. Composition of peripheral blood progenitor cell components collected from healthy donors. Transfusion 1997; 37:411–417. © 1997 John Wiley & Sons. Reproduced with permission of John Wiley & Sons.)