Читать книгу Transfusion Medicine - Jeffrey McCullough - Страница 182

PBSCs can be collected using the Terumo Spectra Optia, the Fresenius Kabi COM.TEC, or the Amicus Separator (Table 6.1). The procedures are the same or similar to those used for MNC collection. PBSCs can be collected from small children [117], but adjustments are necessary in priming the system and managing the patients. Studies comparing the CD34+ cell yield of different apheresis instruments found general equivalency between the collection devices [114, 118, 119].

For normal donors, the usual skin preparation, venous access, needles or catheters, solutions, and software are used. Blood flow rates of 40–80 mL/min are used depending on the donor’s venous access and blood flow tolerance. The MNC collection procedures involve processing 10–15 L of blood over 3 to 4 hours, although usually a larger volume of blood is processed to increase the PBSC yield [114]. There may be recruitment of CD34+ cells during extended apheresis up to 40 L over 5 hours. However, it is not clear that the CD34+ cell levels remain stable or increase (recruitment) during apheresis of normal donors, and so most centers process 15–18 L of blood, and this usually provides a suitable dose in one or two procedures.