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Biosurfactant production in a bioreactor is a complicated process involving the close monitoring of the conditions involved in it. First of all, it is crucial to choose a suitable culture method (i.e. Continuous, Batch, or Fed‐batch), which depends on the type of organism, product, and type of bioreactor [67–69]. Atlić et al. [70] reported that every culture method has specific biomass kinetics, substrates, or final product or byproduct concentrations (Figure 3.2).

Figure 3.2 Key stages used for bioproduct formation in bioreactor.

For example, researchers [71] described an advanced method for surfactin production in the stirred‐tank bioreactor with the help of Aureobasidium pullulans LB 83. Their group used a centrally ordered facial model intending to evaluate the impact of aeration levels (0.1–1.1 per min) and sucrose concentrations (20–80 g/l) on total biosurfactant productivity. Their results reported an increase in tensoactivity of 8.05 cm in an oil spreading test and productiveness of 0.0838 cm/h when all parameters were used at high levels.