Читать книгу Transfusion Medicine - Jeffrey McCullough - Страница 166

The Amicus Separator operates using a separation chamber and a separate component collection chamber (Figure 6.1). The centrifuge chamber design contributes to the fluid dynamics and component separation efficiency. Platelet‐rich plasma is continuously elutriated from the separation chamber, followed by further separation into plasma and platelets in the collection chamber. The Amicus Separator can be used to collect platelets [26–28], PBSCs [29], or a combination of red cells, platelets, and plasma [30]. It can also be used to perform therapeutic apheresis procedures, such as therapeutic plasma exchange, RBC exchange, and photopheresis. In plateletpheresis, the Amicus Separator produces about 3.5 × 10¹¹ platelets in 43 minutes [31]. For collection of PBSCs from patient‐donors stimulated by chemotherapy and granulocyte colony‐stimulating factor (G‐CSF), approximately 1.3 × 10¹⁰ mononuclear cells (MNCs) and 1.4 × 10⁸ CD34+ cells can be obtained from an 8‐L blood processing procedure [32]. When concurrent red cells, platelets, and plasma are collected, the procedure produces 198 mL of red cells, 3.9 × 10¹¹ platelets, and 198 mL of plasma in 74 minutes [30]. The red cells can be stored the usual 42 days when they are resuspended in the additive solution AS‐1 (Adsol).

Figure 6.1 Flow pathway and blood separation of the Fresenius Kabi Amicus Separator. Recirculation of plasma within the chamber increases the efficiency of separation. White circles represent red blood cells; dark circles represent platelets. PRBC, packed red blood cells.

(Source: Courtesy of Fresenius Kabi, Inc.)