Читать книгу Transfusion Medicine - Jeffrey McCullough - Страница 174

The separation between granulocytes from the upper layer of red cells is poor because the density of granulocytes is similar to that of some red cells. Although several agents can be used to sediment red cells in vitro, HES is used because it is licensed in the United States for in vivo use and is not associated with unacceptable reactions or alteration of coagulation tests. The granulocyte yield is doubled when HES is added to the leukapheresis system by constant infusion [63–65]. Several studies of the effects of HES established that the nature and incidence of reactions are acceptable for use on normal donors, the potential for blood volume overload when administered to normal donors can be easily managed during the procedure, there is no adverse effect on laboratory values or platelet or granulocyte function, and there are no adverse long‐term effects. Pentastarch has a shorter in vivo half‐life than HES and can also be used in leukapheresis [66, 67].