Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 178

Limited success has been reported for callus initiation from anthers and ovules of pineapple although there is a high genotype specificity (Benega et al., 1996, 1997a). Irradiation of pineapple pollen has demonstrated a dose and genotype response (Benega et al., 1997b), but the use of irradiated pollen to produce haploid seeds has not been reported.

A method for developing polyploid pineapple from colchicine-treated callus was described by Nagotomi et al. (1997). This technique might be useful for producing doubled haploids from callus derived from anthers or ovules.

The techniques for production of haploid and double haploid pineapple cultivars do not appear to be available to use in breeding programmes. Techniques to improve the success of selfing are likely to be easier and less expensive. In addition, the viability of completely homozygous pineapple plants is questionable given the observed loss of vigour after even limited selfing (Sanewski et al., 2011).