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5.1.2. Organogenesis

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Induction of organogenic cultures has been reported (Arora and Singh, 1978; Litz et al., 1983; Anandan et al., 2011). Shoots were regenerated from callus that developed on cultured seedling stem explants (Arora and Singh, 1978) and from laminae and midribs of papaya cotyledons cultured on modified half-strength MS medium and 0 - ~70 μM BA and NAA (Litz et al., 1983). Shoot buds were induced on cultured sections of epicotyls of an Indian papaya cultivar, ‘Co7’, on MS medium supplemented with 0.5–10 μM thidiazuron (TDZ) (Anandan et al., 2011), thereby avoiding an intermediate callus phase (Arora and Singh, 1978; Litz and Conover, 1982; Litz et al., 1983; Fitch, 1993; Yu et al., 2003).

Biotechnology of Fruit and Nut Crops

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