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According to Rosenberg and Ron [46], these structures consist of lipid molecules in attachment with a polypeptide chain. Antimicrobial action has been shown by many of the biosurfactants against various bacteria, algae, fungi, and viruses. The antifungal and antibacterial property of the lipopeptide, iturin, produced by B. subtilis have been reported by Besson et al. [55] and Singh and Cameotra [13], respectively. A study conducted by Nitschke and Pastore [56] shows lipopeptide activity even after autoclaving between pH 5 and 11 and with a shelf life of six months at −18 °C.