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The biosurfactants production is either enhanced or inhibited by the reaction conditions. Therefore, for the large‐scale production of the desired biosurfactants, it is important to constantly upgrade the bioprocess as the item might be influenced by changes in reaction conditions, i.e. temperature, pH, air circulation, or unsettling speed. Most of the synthesized biosurfactants provide their best performance in a temperature range of 25–300 °C. A change in the biosurfactant composition can occur during temperature variations. Zinjarde and Pant [58] in their study on Yarrowia lipolytica illustrated the best biosurfactant production at pH 8.0, i.e. at regular ocean water pH, which is also the natural surrounding pH of Y. lipolytica. Rhamnolipid production by Pseudomonas sp. occurred at its most extreme pH (6–6.5) and decreases above pH 7.