Читать книгу Biomolecular Engineering Solutions for Renewable Specialty Chemicals - Группа авторов - Страница 26

Transformation is the most commonly used method in cloning. In this simple technique, the DNA from the surrounding is taken up by the competent cells. Some of the host cells like E. coli, yeast, and mammalian cells are not naturally competent. These cells are made competent chemically so that they can easily take external DNA. Calcium and magnesium chloride in specific concentrations in cold condition can make E. coli cells competent.