Читать книгу Biomolecular Engineering Solutions for Renewable Specialty Chemicals - Группа авторов - Страница 36

Cloning vectors are planned in such a way that insertion sites are present in between the genes in the vectors, often antibiotic resistance gene. Vector carrying ampicillin resistance gene confers resistance to ampicillin to the cells in which it will be transformed to. Transformed cells when grown in agar plates containing ampicillin shows resistant to the antibiotic and are capable to grow. But this does not guarantee the presence of gene of interest in the vector or the vector is recombinant. Ampicillin resistance gene become disrupted in vector having gene of interest. Thus, the transformed cells having self‐ligated vector grows on ampicillin agar plate, whereas the cells having recombinant vector does not show growth. The recombinant cells can be selected from the non‐recombinant one through replica plating method.