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The artwork of cloning is to determine the precise transformed cell that consists of the cloning vector with the gene of interest (referred to as a recombinant cell). It is also very important to find the recombinant cells from the culturing transformed petri plate where nonrecombinant (containing the vector without insert DNA) transformed cells are also present. If the final goal is achieved, that means our desired gene of interest is inserted into the vector then it will be called as clone. Direct selection and selection from gene library are the two basic concepts for selection procedures. Direct selection involves designing of experiment in such a way that transformation takes place only for the clones containing specific gene of interest. Selection happens on the plating‐out stage of the transformation where the colonies are grown on agar plates. It is the preferred technique because of the ease and unambiguous in nature. Selection from gene library involves initial “shotgun” cloning test, to produce a clone library representing all or most of the genes present inside the cell, observed through evaluation of each individual clones to identify the correct one.