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3.3.2.3.5.2 Fermentation

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Separate hydrolysis and fermentation (SHF), a two-step method for the processing of butanol, whereas saccharification and fermentation (SSF), a one-step process carried with rice straw [110]. They have claimed that an SSF was more effective than an SHF, notably due to the time reduction from improved productivity. Wirawan et al. [111] showed ethanol production by continuous separate hydrolysis and co-fermentation (SHcF) and simultaneous saccharification and co-fermentation (SScF) from alkaline pretreated sugarcane bagasse using Zymomonas mobilis (PVA immobilized cells) and Pichia stipitis (suspended cells). The ethanol yield and productivity of the SScF fermentation is higher than the SHcF process. S. cerevisiae an C. tropicalis produced ethanol from xylose and glucose without intrusion from H2O2-pretreated corn stover (CS) through SScF [112].

Consolidated bioprocessing (CBP) is a combination process of hydrolysis and fermentation. In modern times, using CBP to transform lignocellulosic biomass into suitable substances has gained considerable research attention to increasing the development of alternative fuels using a cost-effective method [113]. The co-culture of C. saccharoperbutylacetonicum with the fungus Phlebia sp. MG-60-P2 under anaerobic conditions in consolidated bioprocessing synergistically derived butanol from lignocellulosic materials and enhanced saccharification [114]. In another study, Singh et al. [115] have extracted docosahexaenoic acids and bioethanol from rice straw biomass by integrated consolidated thermoanaerobic and marine thraustochytrid bioprocessing. A semi-continuous cycling system possessing self-cycling fermentation (SCF) is used to detect the beginning of the stationary phase; once half of the broth volume has been seen, it is immediately collected then supplemented with a freshly prepared broth to begin the next stage [116, 117]. SCF can improve the yield and efficiency of products. Applying SCF to ethanol production improves efficiency dramatically and enhances the cellulosic ethanol industry [116].

Renewable Energy for Sustainable Growth Assessment

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