Читать книгу Pathology of Genetically Engineered and Other Mutant Mice - Группа авторов - Страница 33

MUTAGENETIX is a database of immunological phenotypes of single gene mouse mutants produced by ENU mutagenesis. It currently contains data generated by the Beutler Lab at the University of Texas Southwestern Medical Center and the Goodnow Lab at the Australian National University and provides access to strains maintained at these centers. The immunological data are very rich and the site contains information about protocols and breeding procedures that are very valuable. Note that incidental, nonimmunological phenotypes are also described for some mutations, such as growth, skin, and endocrine phenotypes. The site is updated regularly (https://mutagenetix.utsouthwestern.edu) [54, 55].

Figure 2.3 IMPC phenotyping pipelines for adult mice (17 weeks) and embryos.

Figure 2.4 The histopathology browser for the IMPC database. Data can be searched for by gene and, where phenotyping of the strain has been completed, users can find data from individual mice, with zygosity of the knockout and in many cases, a diagnostic assessment with lesion and anatomical location derived from the MPATH and MA ontologies and used combinatorially. There are also estimates of severity and microanatomical qualifiers. The use of the ontologies in this way is described in Elmore et al. [49]. Selected hematoxylin and eosin stained images are available.