Читать книгу Genomic and Epigenomic Biomarkers of Toxicology and Disease - Группа авторов - Страница 73

Cytological miRNA examination of pleural effusion is a very challenging task: to distinguish MM from reactive mesothelial cells (RMCS), even if combined with in situ hybridization staining or immunofluorescence, is quite difficult. Cappellesso et al. (2016) screened and verified the differential expression of miRNA-19a (miR-19a, miR-19b), miR-21, and miR-126 in MM cell lines and mesothelioma patient tissue samples. The diagnostic sensitivity and specificity are both over 80%. The combined use of miR-21 and miR-126 can make the diagnostic sensitivity and specificity reach 86% and 87% respectively. In pleural effusion cells, combining miR-143, miR-210, and miR-200c could differentiate MPM with an area under the curve (AUC) of 0.92 (Birnie et al. 2019).