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Different histopathological subtypes have great influence on the curative effect and prognosis of MM, so correctly distinguishing histopathological subtypes is also an important part of the diagnosis of MM. Researchers have studied the difference of miRNAs expression in different tissue subtypes of MM, hoping to apply it to the tissue classification of MM.

Busacca et al. (2010) analyzed miRNAs expression in two MM cell lines (MPP-89 and REN) and formalin-immobilized tumor samples using microarray and RT-qPCR methods. They found that there were seven kinds of miRNAs (miR-17-5p, miR-21, miR-29a, miR-30c, miR-30e-5p, miR-106a, and miR-143) with significant differences in expression, and it can distinguish the three tissue subtypes of MM tumors. The specific expression levels are high expression in epithelial MM, biphasic MM, and low expression in sarcomatous MM. Guled et al. (2009) also compare and analyze that expression of miRNAs in different subtypes of MM tissue. Seven miRNAs were specifically expressed in epithelial MM (miR-135b, miR-181a-2*, miR-499-5p, miR-517b, miR-519d, miR-615-5p, and miR-624), five miRNAs were specifically expressed in biphasic MM (miR-218-2*, miR-346, miR-377*, miR-485-5p, and miR-525-3p), and three miRNAs were specifically expressed in sarcomatous MM (miR-301b, miR-433, and miR-543). Tese miRNAs were specifically expressed in tissue subtypes.