Читать книгу Genomic and Epigenomic Biomarkers of Toxicology and Disease - Группа авторов - Страница 79

Malignant tumors have the characteristics of metastasis and invasiveness. They can metastasize from the primary site of the tumor to distant ones and invade adjacent tissues or organs. At the cellular level, they show the ability of cell migration and invasion. Identifying metastasis-related factors and understanding their role in the metastasis mechanism are helpful processes for the treatment of MM. Studies have found that the upregulation or deletion of some specific miRNAs makes cancer cells have potential metastasis ability. Fassina et al. (2012) used biphasic MM cell line MSTO-211H for migration and invasion experiments, and found that overexpression of miR-205 can inhibit the migration and invasion of tumor cells. Similarly, an in vitro chemotaxis test—a Boyden chamber assay—has been used to detect that the migration and invasion ability of LP-9 cells transfected with miR-34 inhibitor is significantly enhanced (Tanaka et al. 2013); but some tumor cell lines (H28, H290 and H2052) have increased their cell migration and invasion ability after miR-34 methylation (Muraoka et al. 2013). When miR-34b/c is transfected into tumor cell lines, the ability of tumor cells to form clones is obviously weakened, and the migration and invasion ability of cells is also obviously inhibited (Santarelli et al. 2011). However, through the transwell cell migration test and the cell scratch test, the migration ability of MSTO-211H and NCI-H2052 cell lines transfected with miR-145 analogue was compared with that of non-transfected miR-145 analogue cells, and it was found that migration was significantly weakened in the transfected group and was not found at all in the NCI-H28 cell line (Cioce et al. 2014).