Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 75

Jazi et al. (2015) developed a protocol for RNA isolation from pistachio that could be used for downstream studies, including real-time PCR and RNA-seq analysis. Several reference genes, including ACT, EF1α, α-TUB, β-TUB, GAPDH, CYP2, UBQ10 and 18S rRNA, were investigated to reveal the most suitable set of reference genes for transcript level normalization under different conditions, i.e. cold, drought and salt (Jazi et al., 2016). EF1α was the superior reference gene in all samples under all tested abiotic treatments. β-TUB was the second most stable gene for samples under cold and drought treatments, while ACT holds the same position in samples analysed under saline conditions.