Читать книгу Biotechnology of Fruit and Nut Crops - Группа авторов - Страница 82

Akdemir et al. (2012) utilized sonication-assisted Agrobacterium-mediated transformation (SAAT) combined with vacuum infiltration to transform pistachio nodes using a vascular-specific promoter of eucalyptus. Green fluorescent protein (GFP) activity was observed in vascular bundles and leaves of putatively transformed pistachio shoots, and PCR amplification of a 858 bp fragment of nptII (neomycin phosphotransferase II) and 326 bp of uidA (β-glucuronidase) genes confirmed gene integration and showed that SAAT in combination with vacuum infiltration and a vascular specific promoter could be used for transformation.